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KMID : 0617319930030010004
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Journal of Pharmacetical Sceiences Ewha Womans University
1993 Volume.3 No. 1 p.4 ~ p.8
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Cytotoxicity of Folkloric Medicine in Murine and Human Cancer Cells
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Abstract
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The whole plants of Selaginella tamariscina, Orostachycis japonicus, the cortex of Ulmus madshurica, and the wood of Alnus japonica have been used as folk medicine for treating cancer. The cytotoxic activity of these plants were tested using a colorimetric tetrazolium assay(MTT assay). S. tamariscina and A.japonica showed mild IC_50 value, comparing with O.japonicus and U.mandshurica. So, MeHO extracts of S.tamariscina and A.japonica were partitioned into CHCI_3, EtOAc and n-BuOH, successively. The CHCI_3, EtOAc and BuOH fractions of S.tamariscina and A.japonica showed low percent of survival against P388 and MKN45 cells respectively, To isolate active components, they were subjected to silica gel column chromatography. Compound I was obtained from EtOAc extracts of S.tamariscina and identified as amentoflavone by chemical and spectral analysis. Amentoflavone inhibited the survival of P388 cells dose dependently, while not clearly inhibited that of MKN4 cells.
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